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chromosome 19  (Addgene inc)


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    Addgene inc chromosome 19
    Chromosome 19, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chromosome+19/pcDNA4%2FTO-ORF66+C393A-2xStrep+(Plasmid+%23131113)/bio_rxiv__2023__07__24__550349-242-6-33
    Average 91 stars, based on 2 article reviews
    chromosome 19 - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A heterozygous CEBPA mutation disrupting the bZIP domain causes MDS disease progression
    Article Snippet: .. A sgRNA targeting C/EBPα gene on chromosome 19 at position 13.11 was designed using an online tool (Trust Sanger Institute Editing database). sgRNA was first phosphorylated, annealed and cloned into pSpCas9 (BB)-2A-GFP (PX458) (Addgene, 48138) following the protocol provided by . .. P3 amaxa kit (Lonza, V4XP-3024) was used to necleofect iPSC (programme DS-150).



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    Representative findings of fluorescence in situ hybridization for α‐actinin4 <t>(ACTN4).</t> Red and green signals indicate the ACTN4 and <t>CEN19p</t> signals, respectively. The figure shows the 4,6‐diamidino‐2‐phenylindone‐counterstained interphase nuclei for each specimen. (A) A case showing amplification as well as a higher copy number of ACTN4 . (B) A case showing increased numbers of both red and green signals, indicating a higher copy number but no amplification of ACTN4 (original magnification ×1000).
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    Representative findings of fluorescence in situ hybridization for α‐actinin4 <t>(ACTN4).</t> Red and green signals indicate the ACTN4 and <t>CEN19p</t> signals, respectively. The figure shows the 4,6‐diamidino‐2‐phenylindone‐counterstained interphase nuclei for each specimen. (A) A case showing amplification as well as a higher copy number of ACTN4 . (B) A case showing increased numbers of both red and green signals, indicating a higher copy number but no amplification of ACTN4 (original magnification ×1000).
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    Image Search Results


    Representative findings of fluorescence in situ hybridization for α‐actinin4 (ACTN4). Red and green signals indicate the ACTN4 and CEN19p signals, respectively. The figure shows the 4,6‐diamidino‐2‐phenylindone‐counterstained interphase nuclei for each specimen. (A) A case showing amplification as well as a higher copy number of ACTN4 . (B) A case showing increased numbers of both red and green signals, indicating a higher copy number but no amplification of ACTN4 (original magnification ×1000).

    Journal: Cancer Science

    Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

    doi: 10.1111/cas.15841

    Figure Lengend Snippet: Representative findings of fluorescence in situ hybridization for α‐actinin4 (ACTN4). Red and green signals indicate the ACTN4 and CEN19p signals, respectively. The figure shows the 4,6‐diamidino‐2‐phenylindone‐counterstained interphase nuclei for each specimen. (A) A case showing amplification as well as a higher copy number of ACTN4 . (B) A case showing increased numbers of both red and green signals, indicating a higher copy number but no amplification of ACTN4 (original magnification ×1000).

    Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

    Techniques: Fluorescence, In Situ Hybridization, Amplification

    Relationship between clinicopathological parameters and copy number gain of ACTN4 .

    Journal: Cancer Science

    Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

    doi: 10.1111/cas.15841

    Figure Lengend Snippet: Relationship between clinicopathological parameters and copy number gain of ACTN4 .

    Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

    Techniques: Dissection, Over Expression

    Kaplan–Meier estimates of (A) extraurothelial recurrence‐free survival; and (B) overall survival for all 168 patients stratified according to the status of copy number gain of α‐actinin4 ( ACTN4 ). Dashed line curves for cases with copy number gain of ACTN4 ( n = 25), and solid line curves for cases without copy number gain of ACTN4 ( n = 143). The two curves are statistically different [ p < 0.0001 in both (A, B)].

    Journal: Cancer Science

    Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

    doi: 10.1111/cas.15841

    Figure Lengend Snippet: Kaplan–Meier estimates of (A) extraurothelial recurrence‐free survival; and (B) overall survival for all 168 patients stratified according to the status of copy number gain of α‐actinin4 ( ACTN4 ). Dashed line curves for cases with copy number gain of ACTN4 ( n = 25), and solid line curves for cases without copy number gain of ACTN4 ( n = 143). The two curves are statistically different [ p < 0.0001 in both (A, B)].

    Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

    Techniques:

    Univariate analysis for predicting EUR‐FS in patients with UUTUC.

    Journal: Cancer Science

    Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

    doi: 10.1111/cas.15841

    Figure Lengend Snippet: Univariate analysis for predicting EUR‐FS in patients with UUTUC.

    Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

    Techniques: Significance Assay, Dissection, Over Expression

    Multivariate analysis for predicting EUR‐FS in patients with UUTUC.

    Journal: Cancer Science

    Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

    doi: 10.1111/cas.15841

    Figure Lengend Snippet: Multivariate analysis for predicting EUR‐FS in patients with UUTUC.

    Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

    Techniques:

    Kaplan–Meier estimates of (A) extraurothelial recurrence‐free survival; and (B) overall survival for all 168 patients stratified according to the status of α‐actinin4 (ACTN4) protein overexpression. Dashed line curves for cases with ACTN4 protein overexpression ( n = 49), and solid line curves for cases without ACTN4 protein overexpression ( n = 119). The two curves are statistically different [ p < 0.0001 in both (A, B)].

    Journal: Cancer Science

    Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

    doi: 10.1111/cas.15841

    Figure Lengend Snippet: Kaplan–Meier estimates of (A) extraurothelial recurrence‐free survival; and (B) overall survival for all 168 patients stratified according to the status of α‐actinin4 (ACTN4) protein overexpression. Dashed line curves for cases with ACTN4 protein overexpression ( n = 49), and solid line curves for cases without ACTN4 protein overexpression ( n = 119). The two curves are statistically different [ p < 0.0001 in both (A, B)].

    Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

    Techniques: Over Expression

    Univariate analysis for predicting overall survival in patients with UTUUC.

    Journal: Cancer Science

    Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

    doi: 10.1111/cas.15841

    Figure Lengend Snippet: Univariate analysis for predicting overall survival in patients with UTUUC.

    Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

    Techniques: Dissection, Over Expression

    Multivariate analysis for predicting overall survival in patients with UUTUC.

    Journal: Cancer Science

    Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

    doi: 10.1111/cas.15841

    Figure Lengend Snippet: Multivariate analysis for predicting overall survival in patients with UUTUC.

    Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

    Techniques: